Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells
نویسندگان
چکیده
منابع مشابه
L-asparaginase exerts anti-leukemia activity
This study aimed to investigate the effects of L-Asparaginase (L-ASP) on leukemia cell proliferation, cell cycle distribution and geminin expression. Jurkat cells were treated with different concentration of LASP for 24 h, 48 h or 72 h. Cell proliferation was analysed by the CCK-8 assay, cell cycle distribution was examined by flow cytometry, and mRNA and protein expression levels of geminin we...
متن کاملIn vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase.
L-Asparaginase (ASNase) is a potent antileukemic enzyme routinely used in the treatment of children with acute lymphoblastic leukemia. As part of investigations of the biological activity of ASNase, we have developed techniques which measure the in vitro and in vivo cell killing ability of ASNase. To study the effect of ASNase on in vitro survival of primary lymphoblasts, bone marrow mononuclea...
متن کاملIn vitro test for sensitivity of leukemic cells to L-asparaginase.
A method is proposed for measuring the cytocidal effect of L-asparaginase on leukemic blood cells as a possible means of screening patients for L-asparaginase therapy. On the average, L-asparaginase was more toxic to blood lymphocytes from patients with chronic lymphocytic leukemia than to lymphocytes from normal blood. A positive sensitivity test was defined as one in which 65% of lymphocytes ...
متن کاملEffect of l-asparaginase and asparagine deprivation on RNA metabolism in mouse leukemia L 5178Y cells in suspension culture.
We analyzed the effect of asparagine starvation and L-asparaginase on RNA metabolism of mouse leukemia cell lines L5178Y, whose growth is dependent on the presence of asparagine, and L5178Y-R, whose growth is independent of the presence of asparagine. The deprivation of asparagine from the medium inhibited cellular protein synthesis by 30 to 40% of the control value in L5178Y cells, but not in ...
متن کاملUse of L-asparaginase in acute lymphoblastic leukemia: recommendations of the Polish Adult Leukemia Group.
L-asparaginase is a hydrolase that catalyzes the conversion of L-asparagine--an endogenous amino acid necessary for the function of some neoplastic cells, such as lymphoblasts. In most human cells deficiency of L-asparagine can be compensated by alternative synthesis pathway through which L-asparagine is produced from aspartic acid and glutamine by asparagine synthethase. Depletion of L-asparag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science Advances
سال: 2020
ISSN: 2375-2548
DOI: 10.1126/sciadv.abc7120